Driving Bioprocess Innovation with ÄKTA Pure Technology

Written By Moran Yadid Ron

Revitalizing Bioprocess Innovation:
Harnessing the Power of ÄKTA Pure Technology at Scinai BioServices

Powering Protein Purification and Process Development

Bioprocess development relies on precision, reproducibility, and smart experimental design. At Scinai BioServices, one of the core technologies enabling our high-quality purification workflows is the ÄKTA pure chromatography system from Cytiva — a trusted, modular platform used by scientists worldwide to purify proteins, peptides, nanobodies, and nucleic acids.

In this blog, we take you behind the scenes of how ÄKTA pure works, why it is considered the gold standard in chromatography, and how it powers innovative process development at Scinai.

What Is ÄKTA Pure?

The ÄKTA pure is a flexible, modular liquid chromatography system designed to support purification workflows ranging from small laboratory quantities to tens of grams of material. It accommodates the full spectrum of chromatography techniques:

  • Affinity chromatography

  • Ion exchange chromatography

  • Hydrophobic interaction chromatography

  • Multimodal chromatography

  • Size exclusion chromatography

Its versatility allows our scientists to run diverse purification strategies on a single platform — an essential requirement in modern biologic development.

How ÄKTA Pure Works

1. Advanced Hardware for Precision Control

ÄKTA pure integrates reliable and configurable hardware components, including:

  • High-precision pumps

  • UV, conductivity, and pH monitors

  • Modular valves and fraction collectors

This ensures consistent, reproducible performance, even in complex purification workflows.

2. UNICORN 7 — The Software Engine

UNICORN 7 software enables:

  • Drag-and-drop method creation

  • Real-time monitoring

  • Automated workflows

  • Easy, intuitive evaluation tools

Its simplicity allows both junior and senior scientists to operate sophisticated chromatography methods with confidence.

3. DoE for Smart, Data-Driven Optimization

The integrated Design of Experiments (DoE) tool allows systematic testing of multiple parameters at once — dramatically improving efficiency and generating deeper analytical insight.

Why Scientists Trust ÄKTA Pure

ÄKTA systems are trusted globally for their:

  • Versatility across chromatographic techniques

  • Modularity and expandability

  • Ease of use via UNICORN software

  • Efficiency through DoE-driven optimization

  • Scalability to ÄKTA pilot and ÄKTA process systems

This makes the platform especially valuable for organizations preparing materials for clinical and GMP production.

Driving Bioprocess Innovation at Scinai

At Scinai BioServices, ÄKTA pure is central to the development of purification processes for nanobodies and other recombinant proteins.

Our scientists use the system to:

  • Develop, compare, and optimize purification methods

  • Rapidly evaluate buffer conditions, gradients, and elution strategies

  • Build strong, statistically validated processes with DoE

  • Transfer optimized methods seamlessly into ÄKTA pilot and ÄKTA process instruments for GMP production

This ensures a smooth transition from research to manufacturing — a critical success factor for biotech partners advancing novel therapeutics.

A Word from Barry Cohen, Downstream R&D Team Manager

“ÄKTA pure gives us the flexibility to efficiently test multiple parameters with DoE, enabling the design of robust processes at lab scale that are both reliable and data driven. These methods can then be seamlessly transferred into larger ÄKTA systems for GMP manufacturing. This integrated approach allows us to accelerate process development, ensure dependable scalability, and consistently deliver meaningful value to our partners.”

Barry Cohen, Downstream R&D Team Manager, Scinai BioServices

Moran Yadid Ron